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Articles by A. Gupta
Total Records ( 3 ) for A. Gupta
  A. Gupta , V.K. Gupta , D.R. Modi and L.P. Yadava
  The cultural and nutrient requirements of Aspergillus niger for production of α-amylase in production media containing different pH, temperature, incubation period, metal ion concentrations, surfactants, carbon sources and nitrogen sources were quantified in present study. The optimum pH, temperature and incubation period for enzyme production were 5.0, 30°C and 5th day, respectively. Of the carbon sources, starch at 0.5% was recorded to be the best carbon source for enzyme production. Peptone at 0.03% was ideal nitrogen source. However, surfactants Tween-80, Triton X-100 and Sodium dodecyl sulphate at 0.02, 0.002 and 0.0002% concentration were most effective for enhancement of α-amylase production. The main objectives of the present study were to use a suitable fungal strain for production of extra cellular alpha-amylase, to determine the time course for the production of alpha-amylase and to study the effects of external substances that may enhance the production of extra cellular alpha-amylase, including metal ions and surfactants.
  A. Gupta and P.C. Sharma
  The clinicopathological and serological characterization of two field isolates of infectious bursal disease virus (IBDV) recovered from vaccinated flocks that had been vaccinated against infectious bursal disease in Hoshiarpur (Punjab) and Hisar (Haryana) has been undertaken. Broiler chicks (26 days old) were inoculated with 105 p.f.u of Hoshiarpur isolate (group I) and 104 p.f.u. of Hisar isolate (group II) by oral plus intraocular routes. Although both field viruses produced lesions characteristic of IBDV, some differences in the nature and extent of damage were observed. Gross changes included: haemorrhages in the skeletal muscles, atrophy of bursa, haemorrhages in the intestine. The major histopathological lesions observed were: depletion of lymphocytes in both cortical and medullary regions of bursa, depletion of lymphocytes and reticular hyperplasia in spleen, coagulative necrosis in kidney, depletion of plasma cells in Harderians gland, and haemorrhagic foci in thymus. Both the isolates induced high levels of neutralizing antibodies in the sera of IBDV inoculated birds at 21 d.p.i. (log10 4.10 and log10 4.25 in group I and II respectively).
  A. Kumar , A. Gupta , S.S. Atwal , V.K. Maheshwari and C.B. Singh
  Karnal bunt disease of wheat caused by Tilletia indica is a designated disease and a limiting factor in wheat export because most countries regulate the Karnal Bunt (KB) pathogen as a quarantine pest. KB pathogen is seed, soil and air borne. The seed must be threshed and examined for KB infection. Therefore, the post harvest management of KB in wheat seed production through mechanical processing is very important. Pre-cleaner and screen grader removed 83.1 and 61.4% of total KB infected seed present in the seed lot and reduced KB infection from 1.42 to 0.24% and 4.27 to 1.65% in 2012-13 and 2013-14, respectively but it is much higher than the permissible limit of 0.05%. Hence, a total of 18 combinations, comprising of three deck slopes (S1-20, S2-2.50, S3-30), three feedings (F1- 5 kg, F2- 10 kg, F3- 15 kg min-1) and two output settings (O1- 45 cm deck width, O2- 43 cm deck width) of specific gravity separator were studied with an objective of getting maximum Karnal bunt free seed per unit of time. Minimum KB infection in final product (0.04 and 0.28%), maximum final output (12.58 and 12.78 kg min-1) and KB free seed recovery per minute (12.58 and 12.75 kg) with 84.1 and 86.4% recovery efficiency has been obtained by the treatment S1F3O1 (slope of deck 20, feeding 15 kg min-1, output deck width 45 cm) in 2012-13 and 2013-14, respectively. Mechanical processing reduced KB infection by more than 93%, depending on the intensity of infection and increased seed quality i.e., seed germination improved by 7.71% and physical purity by 2.41%.
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