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Articles by A. Gholamhoseinian
Total Records ( 5 ) for A. Gholamhoseinian
  A. Gholamhoseinian , N. Ghaemi and F. Rahimi
  Catalase (EC. 1.11.1.6.H2O2. oxidoreductase) has been found in all aerobic organisms. Most of the work preformed on this enzyme obtained from mammalian, bacterial and fungal sources as there is less information about plant catalases. Partial purification of catalase from Brassica oleracea capitata (Cabbage) and its kinetics was studied. To this intention, freshly harvested cabbage leaves freezed in liquid nitrogen, reduced to small pieces and blended. The extraction with 0.1 M Na2 HPO4 buffer solutions have performed. The filtrate after centrifugation half-saturated with solid Am-Sulfate (A.S) then 35% saturated with solid A.S. After the partially purified enzyme dialyzed, the extract was eluted from a sephadex G-200 column equilibrated with phosphate buffer. The enzymatic activity was observed in only one peak. The optimal pH of the cabbage leaf catalase was 7-8. When the concentration of stabilized catalase increased, the reaction rate increased concomitantly. The substrate was not inhibitory to the reaction rate up to 0.1 M of H2O2 concentration. In this study Vmax and Km of cabbage leaf catalase was 31.12 μM min-1 and 25.5 mM, respectively.
  A. Gholamhoseinian , H. Fallah , F. Sharifi-Far and M. Mirtajaddini
  Alpha 1,2-mannosidase is a key enzyme in N-glycan processing in the endoplasmic reticulum (ER) and Golgi apparatus, have been one of enzyme targets in the development of anti cancer therapies. One hundred species of plants with known and unknown medicinal properties were collected and botanically identified. Methanolic and aqueous extracts prepared by maceration method. Enzyme inhibitory effects against a-mannosidase was determined spectrophotometrically at pH 4.5 and 25 °C using 0.5 mM p-nitrophenyl- α-D-mannopyranoside as the substrate and 1 units mL-1 Jack bean alpha mannosidase in 0.02 M citrate buffer. Among 200 extracts, ten extracts showed more than 20% inhibitory activity on alpha mannosidase; Punica grantum, Damask rose and Quercus infectoria among them showed more than 40%. The kinetic study of the enzyme showed that the inhibition mechanism of the three more active extracts were non competitive. Under the control condition Km value for the enzyme was 1.59 mmol and Vmax was 0.039 mmol min-1 Vmax in presence of 4 μg mL-1 Punica grantum, Damask rose and Quercus infectoria extracts were 0.020, 0.022 and 0.025 mmol min-1, respectively. The data indicated that these plants are good candidates for therapeutic use and deserve to purify the active agents effective against α- mannosidase. Further in vitro and in vivo studies are needed to reveal the actual effectiveness of each of them.
  A. Gholamhoseinian , B. Shahouzehi and F. Sharifi-far
  Pancreatic Lipase (PL) is the most important enzyme in digestion of triglycerides. One of the strategies in prevention or treatment of obesity is altering metabolism of lipids by inhibition of dietary fat absorption. One hundred plant extracts were prepared and botanically identified. The air dried plants were extracted with methanol. Anti lipase activity of each plant was determined by turbidimetric assay. Quercus infectoria, Eucalyptus galbie, Rosa damascena and Levisticum officinale showed more than 50% inhibition on the enzyme activity. Kinetic study of the enzyme was performed in the presence of effective extracts. Levisticum officinale showed mixed inhibition and Rosa damascena, Quercus infectoria and Eucalyptus galbie showed non-competitive inhibition by double-reciprocal Lineweaver-Burk plot analysis. Under the controlled condition, Km value for enzyme was 0.3 mM and Vmax was 0.078 mM min-1; Vmax in the presence of Quercus infectoria, Eucalyptus galbie, Rosa damascena and Levisticum officinale extracts were 0.051, 0.056, 0.049 and 0.068 mM min-1, respectively. Because of mixed inhibition, Levisticum officinale showed a different Km value of 0.617 mM. Further studies needed to elucidate the effectiveness of these active extracts in vivo and attempt should be made to purify their active components to be used as safer and cheaper therapeutic agents in future.
  A. Gholamhoseinian , B. Shahouzehi and F. Sharifi-Far
  Beta-hydroxy-beta-methylglutaryl coenzyme a reductase (HMG CoA reductase) is the key enzyme in cholesterol biosynthesis. Inhibition of this enzyme reduces the synthesis of cholesterol and could be used in the management of coronary artery disease. Drugs used for the management of cholesterol biosynthesis have showed some side effects that are cause of new trends in the nature. This study was designed to find new HMG CoA reductase inhibitors from natural resources. One hundred plants were botanically identified and their methanol extracts were prepared. Anti HMG-CoA reductase activity of the extracts were determined spectrophotometrically by NADPH oxidation, using HMG-CoA as the substrate. Quercus infectoria, Rosa damascena and Myrtus communis extracts showed more than 50% inhibitory effect on the enzyme activity and 21 extracts showed an inhibitory effect between 30-50 percent on activity of HMG-CoA reductase. Kinetic study of the enzyme was performed in the presence of two concentrations of the effective extracts (0.05 and 0.15 mg mL-1). These active extracts showed non-competitive inhibition by Lineweaver-Burk plot analysis. Under the standard condition, Km value for enzyme was 0.6 mM and Vmax value was 0.011 mM min-1. When 0.15 mg mL-1 of extracts of Quercus infectoria, Rosa damascena and Myrtus communis were used the Vmax values of 0.0041, 0.0031 and 0.0028 mM min-1 were obtained, respectively. Therefore, purification and characterization of their active constituents and in vivo examination of these active extracts, is necessary in order to be used as safer therapeutic agents in the future.
  A. Gholamhoseinian , B. Shahouzehi , S. Joukar and M. Iranpoor
  Hyperlipidemia is the cause of many complications in the human societies. In this study, the effect of methanol extracts of Quercus infectoria (QI) galls and Rosa damascena (RD) Mill flower were studied on lipid profile and atherosclerotic plaques formation in hyperlipidemic rabbits. Thirty-six New Zeland white rabbits randomly divided into 6 groups as control (I), hyperlipidemic (II), hyperlipidemic+QI (III), hyperlipidemic+RD (IV), +Atorvastolin (V) and hyperlipidemic+Orlistat (VI) and were fed with high fat diet (0.5% cholesterol and 16% hydrogenated vegetable oil) for 45 days. At the end of the study period, lipid profile and plaque formation were assessed. Total Cholesterol (TC), Low Density Lipoprotein (LDL) and Triglyceride (TG) levels were significantly increased in hyperlipidemic group compared with control group (p<0.001). Methanol extract consumption of Quercus infectoria significantly decreased plasma levels of TC, TG and LDL (p<0.001). It also decreased plaques formation in semi lunar valve and thoracic aorta. Rosa damascena mill flower methanol extract moderately decreased the levels of TC, TG, LDL and plaques formation but it was not significant. HDL levels and weight of animals did not show significant difference among groups. Based on the doses used in this study, our finding indicated that QI but no RD methanol extract has anti atherogenic and hypolipidemic activities.
 
 
 
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