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Articles by A. Bahador
Total Records ( 3 ) for A. Bahador
  Fatolahzadeh B. , P. Maleknejad , A. Bahador , H. Peeri- Dogaheh , M. Y. Alikhani and R. Radmanesh-Ahsani
  Pleural fluid samples from patients with exudative effusion who were diagnosed with tuberculous pleuritis are examined using a new designed primer set based on IS1081 gene (IS1081-PCR) and rpoB-PCR. The PCR results are compared with the results of the sample cultures, using Loewenstein-Jensen (LJ) medium and Ziehl-Neelsen (ZN) staining. Of 78 cases that were confirmed as tuberculous pleuritis by histopathology, supported by sputum culture, biochemical markers (adenosine deaminase, gamma interferon and tumor necrosis factor), radiographic and clinical data, 61 (78.2%) were positive by IS1081-PCR, 43 (55.1%) by rpoB-PCR, 17 (21.7%) by culture and 3 (3.8%) by ZN stain. When IS1081-PCR test results were compared with the confirmed culture, the sensitivity, specificity, positive predictive value and negative predictive value for the IS1081-PCR were 94.1, 55.7, 37.2 and 97.1%, respectively. The corresponding values for the rpoB-PCR were 94.1, 26.2, 26.2 and 94.1%, respectively. When tests results were compare with the confirmed radiographic, histopathology, biochemical markers and clinical diagnosis of tuberculous pleuritis, the IS1081-PCR assay is more sensitive, specific and reliable than both rpoB-PCR assay and culture.
  S. Mahmoudi , H. Abtahi , A. Bahador , G. Mosayebi and A.H. Salmanian
  Streptokinase (SK) is a potent plasminogen activator with widespread clinical use as a thrombolytic agent. In this study, we produce high level expression of recombinant streptokinase in E. coli by expression vector pET32a. Genomic DNA of streptokinase gene (SKC) was extracted, then amplified by polymerase chain reaction (PCR) method and sub-cloned to prokaryotic expression vector pET32a. Escherichia coli BL21 (DE3) pLysS were transformed with pET32a-skc and gene expression was induced by IPTG. The expressed protein was purified by affinity chromatography by Ni-NTA resin. High concentration of the recombinant protein obtained from the single-step purification by affinity-chromatography (Ni-NTA). The yield of recombinant streptokinase was nearly 470 mg L-1 of initial culture. Our data showed that production of recombinant streptokinase improved by pET32a in Escherichia coli.
  B. Geramizadeh , S.A. Malek-Hosseini , H. Salahi , A. Bahador and S. Nikeghbalian
  Hepatoportal sclerosis (HPS) is one of the causes of noncirrhotic portal hypertension. In most patients, hepatic synthetic dysfunction does not occur; rarely they may require liver transplantation. In this study, we have reported the clinicopathologic characteristics of 3 patients diagnosed with HPS after examination of the explanted liver. Small liver volume, significant portal fibrosis, and phlebosclerosis may contribute to hepatic synthetic dysfunction in patients with HPS.
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