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Articles by A. Baghizadeh
Total Records ( 2 ) for A. Baghizadeh
  M. Jorjandi , G.H. Shahidi Bonjar , A. Baghizadeh , G.R. Sharifi Sirchi , H. Massumi , F. Baniasadi , S. Aghighi and P. Rashid Farokhi
  Problem statement: Soil Actinomycetes particularly Streptomyces spp. have showed antagonistic activity against wide range of plant pathogens. In the recent decades they have attracted high interests as biocontrol agents. Onion neck rot or gray mold caused by Botrytis allii have imposed economic post harvest damages to onion bulbs and decreased its storage durability and market value.
To investigate for biocontrol means against the pathogen, antagonistic activity of 50 isolates of soil actinomycetes were assayed through agar disk method and dual culture bioassays. Active isolates were exposed to chloroform for detection of antibiotic. Minimum Inhibitory Concentration (MIC) value and solubility of active crude extract in organic solvents were determined for Streptomyces isolate No. 347 which showed a unique and stable property of inhibiting Botrytis allii. To investigate the antagonistic effect of Streptomyces isolate No 347 on control of onion gray mold, four different treatments were tested by means of Tukey HSD test.
From the tested isolates, 13 showed anti gray mold activities. Exposure of active isolates to chloroform revealed that Streptomyces isolates No 347, 263 and 350 retained their antifungal activities. The active metabolite(s) of Streptomyces isolate No 347 was polar, soluble in H2O but insoluble in chloroform and methanol. MIC of the crude was determined as 0.05 mg mL-1 against B. allii. Stability of the active crude in distilled water at room temperature (12-30°C) was about 6 months. Statistical studies indicated that Streptomyces isolates No 347 can decrease losses of neck rot with significant level (p<0.05).
The future goals include investigation of the antifungal genes in active isolates as candidates for genetic engineering of onion for increased tolerance against B. allii.
  F. Baniasadi , G.H. Shahidi Bonjar , A. Baghizadeh , A. Karimi Nik , M. Jorjandi , S. Aghighi and P. Rashid Farokhi
  Problem statement: High level of biosafety and non adverse effects on the environment of biocontrol strategies of pest management, are priorities of tomorrow's world agriculture. Actinomycetes are active biocontrol agents due to their antagonistic properties against wide range of plant pathogens particularly fungi. Fungal pathogens are liable for a big part of damages in agriculture economy.
In the present research antifungal bioactivity of 50 isolates of Actinomycetes collected from soils of Kerman province of Iran was investigated against Sclerotinia sclerotiorum (Lib.), the causal agent of stem rot in sunflower, through agar disc method and dual culture bioassays. The Streptomyces isolate No. 363 was propagated in submerged cultures and active crude was prepared upon which several biological characterizations performed. Greenhouse studies were achieved to confirm laboratory results.
Among the tested Streptomyces isolates, 10 isolates revealed antagonistic properties in dual culture procedure from which isolate No. 363 showed highest bioactivity. The active metabolite of Streptomyces isolate No. 363 was polar and well soluble in H2O. Using agar-disc method, progressive growth of the pathogen was highly reduced by the antagonist through exhibiting ability to constitute fungus-free zones of inhibitions. The results indicated that isolate No. 363 was a proper candidate for field biocontrol studies.
Results may open a horizon for production of resistant transgenic plants having antifungal properties originated from biologically active Streptomyces spp. recognition and production of effective metabolite(s) of Streptomyces spp. which was responsible for antifungal activities will be our commercial goal due to rich reserves of soil borne Actinomycetes in Iran.
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