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Articles by A. Hoque
Total Records ( 3 ) for A. Hoque
  A. Hoque , A. Nahar , M.A. Razvy , M.K. Biswas and A.H. Kabir
  Three varieties of water chestnut (Trapa quadrispinosa, T. bispinosa and T. acornis) collected from Rajshahi, Naogaon, Natore have been used in this study. Embryonal explants were found to be best for callus induction on the medium containing half strength MS salts in combination of 2.5 mg L-1 2,4-D and 1 mg L-1 BA in all three varieties. For survival of explant 0.1% HgCl2 having 7 minutes of treatment period was found to be best. Addition of CH (2.0 mg L-1) and PG (2.0 mg L-1) proved to be effective to overcome the phenolic oxidant during callus induction. Callus derived plantlet formation was being a difficult task in water chestnut. Liquid half strength MS medium supplemented with 1.1 mg L-1 BA and 0.5 mg L-1 NAA has been proved to be best for shoot proliferation in all the varieties. Moreover, addition of 0.5 mg L-1 GA3 with aforesaid combination highly stimulated the axillary shoot elongation of Trapa sp. Rooting of produced shoot showed highest percentage in a liquid half strength MS medium containing 1.1 mg L-1 IBA. This in vitro plantlet were then transferred and acclimatized in plastic pots under favorable humidity and temperature. After 4 weeks of acclimatization callus derived plantlets were established successfully in a water chestnut field. It was found that all the plantlets were disease free and the survival rate was almost 95%.
  A. Hoque , T. Anai and S. Arima
  Molecular characterization and genetic variation patterns between 18 varieties of water chestnut (Trapa sp.) were investigated through RAPD markers. PCR amplifiable DNA was isolated using the CTAB method and amplified fragments were obtained from 20 random 10-mer and 20 random 12-mer primers. Genetic distances among the different varieties were analyzed with a UPGMA-derived dendrogram. Similarity matrix showed the similarities between varieties ranged from 0.25 to 1.0. The dendrogram results suggested that the European varieties were in the same cluster group. Japanese varieties were divided into three clusters group. Indian varieties were in the separate cluster group. Korean and Japanese small were in the same cluster group. Chinese 1~7 and Japanese medium and Kobe-large were in the same group but have sub-group. Genetic distance of Kobe small is high and most devient from other varieties and made separate cluster group.
  M.K. Biswas , M.A.A. Mondal , M.G. Ahmed , A. Hoque , M.M. Hossain and R. Islam
  Genetic variability and heterosis for eight quantitative traits were evaluated in seven parents and ten hybrids. The hybrids were derived from a 5x2 line x tester mating design. In general high component of variation and coefficient of variability were observed for most of the characters. The highest component of variation, coefficient of variability and heritability were noticed in PW, TW 60 and TN 60. Characters those with high genetic variability and genetic advance were considered to be important for selecting the desirable parents. Heterosis was worked out over mid parent, better parent and standard parent. Combinations AU/13, LP/13, PT/13 and LS/13 for PH; combination PT/13 and LP/13 for TN 60 were significant for mid parent, better parent and standard parent heterosis. The best heterotic combinations for TW 60 were LP/13, LS/13 and LSB/13 which recorded 990.28, 1115.28 and 648.61% standard heterosis respectively and can be utilized for hybrid development.
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