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Articles by A. Bazli Syah
Total Records ( 1 ) for A. Bazli Syah
  L. Chin Ming , R. Nurliyana , A. Bazli Syah , M. Noor Azizah , H. Li Sim and M.Y. Hirzun
  Microalgae are being studied aggressively lately as a potential feedstock for biofuel and other valuable compound production. In this study, marine microalgae were collected at Fisheries Department of Terengganu in Malaysia which were then being isolated, purified and identified using rDNA approach. For the molecular identification analysis, several DNA extraction methods were investigated in order to obtain high quality algal DNA. Chloroplast genomes were amplified successfully using eight sets of universal chlorophyte primers from the genomic DNA extracted. These amplified fragments were then purified and ligated into pGEM-T vector which was subsequently cloned into E. coli competent cell. Plasmids DNA of the selected transformed cells were extracted and prepared for sequencing. The sequences were analyzed and compared with other strains in NCBI database. Based on the chloroplast sequences, the collected strain is identified as Parachlorella kesslari. As for the analysis of biochemical composition of this strain, the result revealed that it composed of 30% lipid, followed by 25% carbohydrate, 15% protein and 8% starch, respectively. Forty percent of the extracted lipid was composed of PUFAs through GC-MS analysis.
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