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Articles by A. Ansary
Total Records ( 3 ) for A. Ansary
  W.H. Himratul-Aznita and A. Ansary
  Periodontal disease is a global public health problem, including in Malaysia. The destruction of the periodontium has been associated with subgingival plaque microflora. Prevotella intermedia has been categorized as a periodontopathic bacteria as it contributes to the development and progression of periodontal disease. Therefore, a study on P.intermediaís virulent properties is important for the understanding of periodontal disease. Thus, the aim of the study is to characterize the virulence effect of the intracellular toxin. In the study, intracellular toxin of P.intermedia from ninety isolates were obtained and injected subcutaneously in male balb/c mice, aged between 8-12 weeks old. The results showed that the intracellular toxin from all P.intermedia isolates were able to cause the development of localized lesions on the skin of balb/c mice when 0.1, 0.2 and 0.3 mL filtrates were used. In addition, it was found that the degree of skin lesions on the balb/c mice was dose dependent as the infective dose was found to correspond to the development of larger lesion accompanied by skin coagulative necrosis. In the second part of the study, the intracellular toxin was heated for 1, 5, 15, 30, 45 and 60 min at different temperatures of 37 and 60 C prior to the infection in mice. It was discovered that the intracellular toxin was not stable and sensitive towards high temperature and is thermolabile when the activity was terminated upon heating to 60 C as none of the injected mice developed skin lesion.
  W.H. Himratul-Aznita , T.B. Taiyeb Ali , K. Sushil , S.L.A. Zainuddin , A.A. Mahmood and A. Ansary
  The study was undertaken with the aims of identifying strains providing virulent toxin and to observe the histological effect of the toxin in mice. Plaque samples were collected from adult periodontitis patients. P. intermedia were recovered from subgingival periodontal pocket with depths of 5 mm or greater. Ninety P. intermedia isolates were identified based on its bacteriological properties, gram staining and biochemical characteristics. The clinical isolates of P. intermedia were assessed for their potential and ability to produce toxin and form skin lesion in balb/c mice. 108, 1010 and 1012 cells mL 1 of bacterial suspension were used in the study. No lesion was observed in mice injected with 108 cells mL 1 and only three P. intermedia with concentration 1010 cells mL 1 were able to induce localized skin lesion in balb/c mice. However, all isolates causes balb/c mice to develop localized skin lesion when 1012 cells mL 1 was used. Infected mice appeared cachectic and the histological effect of the skin lesion showed that all lesions were localised at the injection site and causes tissue damage with skin necrosis and hair loss.
  W.H. Himratul-Aznita and A. Ansary
  The study was undertaken with the aims of identifying Prevotella intermedia virulent strains from adult periodontitis and to detemine its virulent gene via molecular analysis. The clinical isolates were assessed for their potential and ability to produce toxin and form skin lesion in balb/c mice. Infected mice appeared cachectic and the histological effect of the skin lesion showed that all lesions were localized at the injection site and causes tissue damage with skin necrosis and hair loss. Prevotella intermedia UMD 5 was the most virulent isolate and thus was selected for shotgun cloning. Partially digested genomic DNA (2-9 kb) from P. intermedia UMD 5 was cloned into the Bam HI site of E. coli pUC 18. The inserted gene for all recombinant clones was expressed in balb/c mice. It was found that only one clone was able to induce localized skin lesion in mice. The cloned gene expressed well by inducing skin lesion in mice when incubated in aerobic environment, but the expression was better in combination of aerobic followed with anaerobic environment, producing bigger skin lesion area in balb/c mice. Thus, the presence or absence of O2 did not suppress the level of toxin production for anaerobic bacterial gene cloned in the aerobic system. Histological effect of the skin lesion showed that lesions were localized at the injection site and causes tissue damage with skin necrosis and hair loss. In addition, the recombinant plasmid was found to be stable in the host system. The clone was further analyzed via restriction analysis and was found to have a DNA insert of 4 kb.
 
 
 
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