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Articles by A. Ahmad
Total Records ( 3 ) for A. Ahmad
  S. Hayat , Q. Fariduddin , Barket Ali and A. Ahmad
  Chloroindole auxins are useful tools in understanding the signal transduction pathways of the auxins. However, their presence is not acclaimed in majority of the plants. This gives an impression that these auxins could be having certain roles in plants enriched with them. Therefore, the seeds of Solanum melongena (L.) cv. Pusa were soaked in 0, 10-8 or 10-6 M aqueous solutions of IAA, 4,Cl-IAA, 5,Cl-IAA, 6,Cl-IAA, 7,Cl-IAA, 4,6,Cl2-IAA and 4,7,Cl2-IAA for 8 h and cultivated in clay pots. All the auxins improved the growth and NR activity compared with water soaked control. 4,Cl-IAA was most prominent in its effect, generating 29, 54, 30, 34 and 47% higher values for shoot and root length, fresh and dry mass and NR activity, respectively, in 15 day old plants. The order of response to various auxins was 4,Cl-IAA>7,Cl-IAA>6,Cl-IAA = 5,Cl-IAA = 4,6Cl2-IAA = 4,7Cl-IAA>Control.
  S.B. Maqbool , A. Ahmad , M.B. Sticklen and S.A. Hashsham
  Polyphosphate kinase gene (ppk) encodes the enzyme polyphosphate kinase (PPK) that is thought to be responsible for the synthesis of polyphosphate (poly-P) in phosphorus accumulating organisms (PAOs). Methods to detect and quantify ppk gene simultaneously may be useful to detect microbial communities containing PPK. The objective of this study was to develop a real time polymerase chain reaction (RLT-PCR) assay for the detection and quantification of ppk gene in activated sludge. RLT-PCR conditions were optimized to amplify a 1.2 kb fragment of known ppk gene sequences in a plasmid and in Pseudomonas aeruginosa using degenerate primers. The protocol was then modified to use with the ABI Prism 7700 sequence detection system using SYBR® Green I for real time quantification of the amplicons. Four activated sludge microbial communities expected to contain varying levels of PAOs were analyzed to quantify the abundance of ppk. The results indicated that communities enriched for PAOs contained 10- to 100-fold higher levels of ppk (103-105 copies of ppk gene per 100 ng of total DNA) than the non-enriched communities (102 or less copies per 100 ng of total DNA). RLT-PCR is indeed a promising method to monitor environmental samples of complex nature and high similarity.
  V. Nissapatorn , C.K.C. Lee , Y.A.L. Lim , K.S. Tan , I. Jamaiah , M. Rohela , B.L.H. Sim , A. Ahmad , S. Hadita , P.W. Lott , K.T. Ng , M.E. Poh , J. Zuliana and A. Khairul Anuar
  A total of 693 HIV/AIDS patients were recruited in this retrospective and descriptive study during April 2003 to December 2004, Hospital Kuala Lumpur, Malaysia. It was found that both genders were shown to be within the same range of age (M = 18-79 vs F = 18-73), while a median age was slightly higher in male (36 years) than female (31 years). The majority of both genders were significantly shown in the age group of 25 to 34 years, but a higher rate was evident in females (42%) (p = 0.001). It was even observed that male patients were mainly Chinese (44.7%) and single (57.3%) (p = 0.001), while the females were Chinese (40.6) and married (83.3%) (p = 0.000). However, the highest numbers of both genders were manifested to be heterosexuals (M = 47.6% vs F = 86.2%), followed by intravenous drug users (M = 39.3% vs F = 6.5%) (p = 0.000). The range of CD4 cell count was 0 to 1799 with a median of 230 cells/mm3. The level of CD4 cell count of < 200 cells/mm3 was significantly found in males (48.5%) and between 200 to 499 and ≥500 cells/mm3 were 36.2 and 26.8% in females, respectively (p = 0.001). Overall, the Toxoplasma seroprevalence was 43.85% where seropositive of anti-Toxoplasma antibody relatively higher in males (50%) than in females (37.7%) (p = 0.015). Toxoplasma seropositivity was thoroughly evident among Malays (168; 51.1%), Intravenous Drug Users (IDUs) and patients with toxoplasmic encephalitis (TE) (p = 0.001). Furthermore, anti-retroviral therapy including HAART was more significantly found in patients with 9 seropositive Toxoplasma when compared to 2 seronegative and unknown serostatus patients (p = 0.025). Seventeen AIDS-related toxoplasmic encephalitis patients were diagnosed at the time of this study, depicting hemiparesis as the most common neurological manifestation in 11 (64.7%) patients, followed by headache and seizure in 6 (35.3%) and 3 (17.7%) patients, respectively. Interestingly, CT scan finding showed mass like structure with multiple (58.8%), ring enhancing lesions (100%), in parietal region (58.8%) and edema (29.4%) in these patients. Overall, the treatment outcome showed that 13 (76.5%) patients had completed treatment with maintenance, whereas, 3 (17.6%) patients were lost to follow up and 1 (6%) patient was transferred to another hospital. No relapse or death case was reported during the time of this study.
 
 
 
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