Abstract: Background and Objective: The purpose of the study was to investigate the influence of laser applied on acupuncture points in endothelial dysfunction on hypertensive obese patients. Materials and Methods: Sixty patients from both genders aged between 40-50 years old were assigned into two groups equal in number. Group A: Received a program of aerobic exercises and laser on acupuncture points 16 J per point and group B: Received a program of aerobic exercises and sham laser (placebo) on acupuncture points. The program was 2 times per week for 24 weeks. Urinary Nitric Oxide (NO) and blood superoxide dismutase (SOD) were measured at the beginning and at the end of the study. Results: The results of study group revealed that there was a significant increase in nitric oxide (81.82%) and superoxide dismutase (78.72%) levels, which means reduction in oxidative stress and improvement in endothelial function. Conclusion: It could be concluded that for hypertensive patients using laser puncture two times per week improved their nitric oxide and superoxide dismutase levels which is important antioxidants in the body. Laser applied on acupuncture points improve general health.