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Turkish Journal of Veterinary and Animal Sciences
Year: 2008  |  Volume: 32  |  Issue: 5  |  Page No.: 353 - 358

Surfactant Proteins A and D in the Seminal Plasma of Stallions

Ayhan ATA, Orhan KANKAVI and M. Akif CIFTCIOGLU    

Abstract: We have previously shown that surfactant protein (SP)-A and SP-D are present in the stallion’s genital system. The aim of the present study was to evaluate the expression and presence of SP-A and SP-D in the seminal plasma and accessory sex glands of stallions. We characterized further the SP-A and SP-D in the seminal plasma of stallions by western blotting and immunohistochemically in accessory sex glands. On one-dimensional SDS-PAGE electrophoresis and western blots, bands for SP-A (34 kDa) and SP-D (43 kDa) in seminal plasma and accessory sex gland tissue extracts were detected. The similarity between biochemical properties (relative molecular masses) of SP-A and SP-D in accessory sex glands and seminal plasma imply that SP-A and SP-D proteins are also present in the seminal plasma and accessory sex glands of stallions.

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