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Turkish Journal of Medical Sciences
Year: 2010  |  Volume: 40  |  Issue: 5  |  Page No.: 715 - 721

Effects of vitamin A and C and melatonin on 3-nitrotyrosine formation in guinea pig heart under lipopolysaccharide-induced stress

Halil YAMAN, Tuncer CAYCI, Melik SEYREK, Emin Ozgur AKGUL, Yasemin GULCAN KURT, Ibrahim AYDIN, Hakan YAREN, Erdinc CAKIR, Omer OZCAN, Behzat CIMEN, Nurten TURKOZKAN and Mehmet Kemal ERBIL    

Abstract: Aim:Bacterial lipopolysaccharide (LPS) triggers synthesis of nitric oxide (NO) and superoxide (O2.-). O2.- can react with NO to produce the powerful oxidant peroxynitrite (ONOO-). The presence of 3-nitrotyrosine (3-NT) in tissues is often used as a marker of ONOO- production. Vitamin A, vitamin C, and melatonin are potent antioxidant molecules. We sought to clarify this issue by examining the effects of vitamin A (15,000 IU/kg/day), vitamin C (600 mg/kg/day), and melatonin (25 mg/kg) on 3-NT formation in heart tissue in a guinea pig model of LPS-induced endotoxemia. Materials and methods: A total of 75 animals were randomly divided into 5 groups (n = 15 animals for each group). 3-NT levels in the heart tissue were determined by high pressure liquid chromatography with a diode array detector. Results: In the group given LPS, 3-NT levels were significantly increased compared with the control, vitamin A + LPS-treated, vitamin C + LPS-treated, and melatonin + LPS-treated groups. In the vitamin A + LPS-treated group, vitamin C + LPS-treated group, and melatonin + LPS-treated group, 3-NT levels were similar to those of the control group. Conclusion: Vitamin A, vitamin C, and melatonin pretreatment significantly prevented 3-NT formation. These agents may offer an advantage in that they could improve the hemodynamics as well as reduce the formation of ONOO-.

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