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Turkish Journal of Botany
Year: 2010  |  Volume: 34  |  Issue: 3  |  Page No.: 233 - 240

Clonal propagation of Dorystoechas hastata via axillary shoot proliferation


Abstract: A protocol for the in vitro seed germination, seedling development, and axillary shoot propagation of Dorystoechas hastata Boiss. & Heldr. ex Bentham is described. By employing sterilised seeds that were transferred onto in vitro media, the effects of various in vitro media, photoperiods, and temperatures on germination were investigated. At the end of these experiments, a final protocol was determined for the in vitro germination of D. hastata seeds. With this protocol, the highest germination rate was obtained with distilled water supplemented with vitamins and 1 mg L-1 of gibberellic acid (GA3), with a 16:8 photoperiod at 24 ± 2 °C. Germinated seeds were transferred onto various in vitro media for further seedling development, and the appropiate medium for seedling development was found to be half-strength Murashige and Skoog medium (MS). In axillary shoot propagation experiments, the highest shoot number per explant was obtained with half-strength MS medium supplemented with 0.5 mg L-1 of N6-Benzyladenine (BA). Solitary shoots, removed from stock cultures, were transferred onto half-strength MS media supplemented with various concentrations of indole-3-butyric acid (IBA) or naphthalene acetic acid (NAA) for rooting. The maximum rooting rate was obtained with half-strength MS medium supplemented with 1 mg L-1 of IBA. Rooted plantlets were gradually acclimatised to the external environment.

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