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Trends in Applied Sciences Research
Year: 2011  |  Volume: 6  |  Issue: 10  |  Page No.: 1206 - 1213

Production and Characterization of Alkaline Protease from Bacillus licheniformis sp. Isolated from Iranian Northern Soils with Ram Horn Hydrolysate

Masumeh Anvari and Gholam Khayati    

Abstract: The aim of this study was the use of ram horn hydrolysate as a substrate for alkaline protease production using Bacillus spp. in submerged fermentation method. Sixty-three bacillus strains were isolated from Iranian alkali soils. Thirty were identified as Bacillus licheniformis. The enzyme solution from these B. licheniformis strains were investigated for their proteolytic activity. When tested on skim milk agar, the enzyme solution of B. licheniformis strain 5 exhibited the highest proteolytic activity (a clear zone with an average area of 480 mm2). The results show that with the addition of RHH to the fermentation medium with a final concentration of 4% (optimal concentration), alkaline protease value reached a maximum value (93 U mL-1) which is 69% higher than that of the control medium. Optimal pH and temperature activity of the alkaline proteases from B. licheniformis strain 5 were found to be at 9 and 50°C, respectively. The protease was unstable and rapidly decreased in activity when heated at 60°C. It was activated by Mn+2, Ca+2. The enzyme activity was strongly inhibited by PMSF, suggesting that it belong to the family of serine proteases.

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