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Trends in Applied Sciences Research
Year: 2007  |  Volume: 2  |  Issue: 5  |  Page No.: 419 - 425

Immobilization of Aspergillus niger Pectinase on Polyacrylonitrile Copolymer Membrane

Ginka Delcheva, Ivan Pishtiyski, Georgi Dobrev and Svetla Krusteva    

Abstract: Aspergillus niger pectinase was successively immobilized on polyacrylonitrile copolymer membrane. The optimal duration of immobilization process and optimal enzyme concentration of 1% (w/v) at pH 3.0 were determined. Immobilized enzyme showed a shift in pH optimum which was at pH 5.0 for the enzyme immobilized on not activated membrane and at pH 5.5 for the enzyme immobilized on membrane activated with glutaraldehyde. Both immobilized enzymes exhibited maximum activity at 60°C. Free pectinase exhibited higher stability than that of the immobilized enzyme at 30, 40 and 50°C. In order to examine the reusability of immobilized pectinase 22 consecutive batch cycles were performed.

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