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Sensors and Actuators B: Chemical
Year: 2009  |  Volume: 139  |  Issue: 2  |  Page No.: 592 - 597

Label-free DNA detection based on a novel nanostructured conducting poly(indole-6-carboxylic acid) films

Guangming Nie, Yan Zhang, Qingfu Guo and Shusheng Zhang    

Abstract: A simple and label-free electrochemical sensor for detection of DNA hybridization event was obtained based on a novel nanostructured conducting polymer, poly(indole-6-carboxylic acid). This precursor polymer with high electroactivity can be easily prepared by direct electrochemical polymerization of indole-6-carboxylic acid in acetonitrile containing tetrabutylammonium tetrafluoroborate. An 18-mer amino-substituted oligonucleotide (ODN) probe was covalently grafted onto the polymer in a one-step procedure. The performance of this label-free electrochemical DNA sensor was investigated by using cyclic voltammetry (CV) as a readout method. This simple sensor can discriminate one-base mismatched and non-complementary ODN sequences and can be regenerated after a dehybridization step. The dynamic determination range for complementary target ODN was from 3.5 x 10–10 mol L–1 to 2.0 x 10–8 mol L–1 and the corresponding detection limit was 5.79 pmol L–1.

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