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Research Journal of Medical Sciences
Year: 2014  |  Volume: 8  |  Issue: 2  |  Page No.: 67 - 72

Hematinic and Anti-Anemic Effect of Thymoquinone Against Phenylhydrazine-Induced Hemolytic Anemia in Rats

Tariq Helal Ashour    

Abstract: Herein, the possible hematinic and anti-anemic effects of TQ supplementation therapy was investigated in a rat model of hemolytic anemia induced by Phenylhydrazine (PHZ). Forty eight adult male Wistar rats (18 rats per group) were randomly classified into: normal control group, PHZ group and PHZ+TQ group. PHZ was injected intraperitoneally at 40 mg kg¯1 on day 0 and 2 additional doses were given at 9 am and 6 pm, on day 1 while TQ (15 mg/kg/day) was given orally. Six rats of each group were sacrificed at days 3, 5 and 8 and their blood samples were collected at each time point for analysis. After 3-5 days of PHZ injection, rats developed acute hemolytic anemia reflected by significant decreases in RBC count, Hemoglobin (HGB) concentration and Hematocrit (HCT) percentage and significant increases in reticulocytes population and serum heme concentration. Interestingly, simultaneous therapy with TQ had significantly reversed these deteriorating effects of PHZ on RBCs, HGB, HCT and heme at each time point of analysis, however, it trended to induce further increase in reticulocytes population. Additionally, TQ therapy significantly reversed the decreases in serum levels of total Glutathione (GSH) and activities of Superoxide Dismutase (SOD) (as indices of antioxidant status) as well as the increases in serum levels of Thiobarbituric Acid Reactive Substances (TBARS) (as indices of lipid peroxidation and oxidative stress) that were induced by PHZ intoxication. The present data suggest the favorable hematinic and anti-anemic effect of TQ therapy on PHZ-induced oxidative stress and hemolytic anemia in rats. This in turn may pave the way to use TQ as part of anemia management.However, further studies are required to confirm this suggestion.

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