Abstract: The aim of this study was to determine the in vivo antioxidant activity of newly formulated Oil in Water (O/W) creams of methanolic extract of Cassia tora L. leaves. O/W creams of methanolic extract of Cassia tora L. leaves was prepared, evaluated and tested for acute dermal toxicity study. The different O/W creams showed good physical characteristics and pass the sensitivity, irritation, grittiness and bleeding test. Exposure of Ultraviolet-B (UV-B) light decreased the reduced Glutathione (GSH), Catalase (CAT) and Superoxide Dismutase (SOD) activities and increased the Lipid Peroxidation (LPO). The oxidative stress induced by exposure of UV-B light in rats was minimized by single dose topical application of different concentrations of O/W creams and Cassia tora Methanolic extract (CTM). Researchers concluded that topical O/W creams and methanolic extract of Cassia tora leaves has potent antioxidant activity.