Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Research Journal of Medicinal Plants
Year: 2016  |  Volume: 10  |  Issue: 5  |  Page No.: 349 - 355

Determination of Stevioside and Rebaudioside A from Simulated Stevia Beverages Using FTIR Spectroscopy in Combination with Multivariate Calibration

Yohanes Martono, Sugeng Riyanto, Sudibyo Martono and Abdul Rohman    

Abstract: Background and Objective: Simulated stevia beverages contain major diterpene glycoside, stevioside and rebaudioside A from Stevia rebaudiana . Methodology: The objective of this study was to develop FTIR spectroscopy in combination with multivariate analysis of Partial Least Square (PLS) regression for determination of stevioside and rebaudioside A in simulated stevia beverages. The PLS calibration was optimized by selecting wave number region capable provided the highest coefficient determination, R2 and lowest Root Mean Square Error Calibration (RMSEC). Results: Finally, the combined wave number range of 1161-2773 and 868-1041 cm–1 using Multiplicative Scattering Correction (MSC) followed by offset correction transformation spectra was chosen for stevioside determination with R2 and RMSEC value of 0.9954 and 3.40%, respectively. Meanwhile, selected wave number region at 791-1839 cm–1 without spectra transformation revealed optimal PLS regression for rebaudioside A determination with R2 and RMSEC of 0.9820 and 2.91%, respectively. Conclusion: The FTIR spectroscopy in combination with multivariate analysis of PLS regression could be used an alternative method for determining stevioside and rebaudioside A in simulated stevia beverages.

Cited References   |    Fulltext    |   Related Articles   |   Back
 
 
   
 
 
 
  Related Articles

 
 
 
 
 
 
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility