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Research Journal of Medicinal Plants
Year: 2013  |  Volume: 7  |  Issue: 2  |  Page No.: 92 - 99

Polymerization Studies of Sickle Cell Hemoglobin Incubated in Aqueous Leaf Extract of Nicotiana tabacum Product

P.C. Chikezie, A.R. Akuwudike and C.M. Chikezie    

Abstract: In vitro studies have revealed that plant extracts interfered and altered the polymerization profile of deoxygenated sickle cell haemoglobin molecules (deoxyHbS). The present study seeks to ascertain the capacity of aqueous extract of N. tabacum to alter and interfere with polymerization of deoxyHbS molecules in vitro. Spectrophotometric method was used to measure level of sodium metabisulphite induced polymerization of deoxyHbS molecule incubated in aqueous extract of N. tabacum for 180 sec. The polymerization profile of deoxyHbS molecules of control and test samples showed increasing level of polymerization with progression of experimental time. At experimental time t>30 sec, level of polymerization of control sample ranged between 60.9±0.76-100±1.05%, whereas, the test samples ranged in the following corresponding order: (N. tabacum) = 0.8 mg 100 mL-1, 65.6±0.93-176.0±4.26%, (N. tabacum) = 1.0 mg 100 mL-1, 75.7±1.07-192.9±5.03% and (N. tabacum) = 2.0 mg 100 mL-1, 135.9±5.04-297.2±19.14%. Activation of deoxyHbS polymerization by aqueous extract of N. tabacum increased in a concentration dependent manner and duration of incubation. Specifically, at t =180 sec, (N. tabacum) = 2.0 mg 100 mL-1 caused highest level of activation of deoxyHbS polymerization (197.2±19.14%); an increase in % polymerization in a ratio of 1:4.5 (approx.) compared with (N. tabacum) = 0.8 mg 100 mL-1 at t = 30 sec. The study showed that aqueous extract of N. tabacum exacerbated polymerization of deoxyHbS molecules in a concentration and time dependent manner. Therefore, N. tabacum in the present experimental form did not exhibit therapeutic potentials for management and alleviation of sickling disorder.

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