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Research Journal of Medicinal Plants
Year: 2011  |  Volume: 5  |  Issue: 1  |  Page No.: 79 - 84

In vitro Antioxidant Activity of Extracts from Eupatorium odoratum L.

S. Amatya and S.M. Tuladhar    

Abstract: The aim of this study was to assess the in vitro potential of ethanolic extracts of various parts of Eupatorium odoratum L. The antioxidant activities of ethanolic extracts of leaf, stem, root and defatted flower parts were evaluated by β-carotene bleaching and 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assays. Leaf and flower revealed the good antioxidant activities in both methods. In β-carotene bleaching assay, ethanol extracts of leaf and flower exhibited the relative antioxidant activity values as 0.95± 0.029 and 0.86±0.05, respectively. The DPPH activity of ethanol extracts of leaf and flower was increased in a dose dependent manner, which was found in the range of 5.65-87.93 and 13.98- 91.4% inhibition, respectively, as compared to butylated hydroxyanisole (23.29-91.05). The IC50 of leaf and flower extracts in DPPH radical was 44.5 and 50.9 μg mL-1, respectively. This study suggests that leaf and flower parts of E. odoratum could be pharmaceutically exploited.

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