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Research Journal of Medicinal Plants
Year: 2011  |  Volume: 5  |  Issue: 1  |  Page No.: 42 - 53

Antioxidant and Antibacterial Activities of Two Combretum Species from Burkina Faso

T.H. Coulidiati, H. Millogo-Kone, A. Lamien-Meda, M. Yougbare-Ziebrou, J. Millogo-Rasolodimby and O.G. Nacoulma    

Abstract: The aim of this study was to determine the phytochemical content and to evaluate the antioxidant and antibacterial activities of the acetone extract from Combretum acutum Laws (leaves) and Combretum sericeum G. Don (aerial part). Acetone extracts were fractionated with n-hexane, ethyl acetate and butanol successively and their bioactivities were also evaluated. Phenolic, tannin and flavonoid contents have been determined using spectrophotometric methods. The antioxidant activity of these plant extracts and fractions has been determined by ABTS, FRAP and DPPH methods. The phytochemical screening revealed the presence of tannins, steroids/triterpenoids, saponins and cardenolides and different contents of flavonoids and anthraquinones in the acetone extracts of both plants. Results obtained from this study also showed that the butanol fractions, with the highest phenolic and tannin contents, exhibited the best antioxidant and antibacterial activities and will be maintained for further investigations. In addition to that, the Minimum Inhibitory Concentrations (MICs) of the extracts and fractions against pathogenic bacteria (5) and serotyped bacteria (5) from American Type Culture Collection (ATCC) were also determined using the agar-well diffusion method. Both plants possess good antioxidant and antibacterial activities.

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