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Research Journal of Medicinal Plants

Year: 2011  |  Volume: 5  |  Issue: 3  |  Page No.: 330 - 337

In vitro Validation and Phyto-constituent Analysis of Turmeric Extract: An Ethnological Alternative for Eye Treatment

S. Singh, B.S. Sathpathy, R.K. Sahoo, E. Subudhi and S. Nayak


The essential oil from the rhizomes of Roma cultivar of turmeric (Curcuma longa) from Orissa was examined for its antimicrobial activity against the pathogens causing eye infections. The purpose of this study was to authenticate the use of turmeric rhizome oil against eye infections so as to giving an approach to formulate turmeric rhizome oil as potential eye drop in place of traditional antibiotics after undertaking its in vivo pharmacological studies. Essential oil from rhizomes of Roma cultivar obtained by hydrodistillation extraction method using clenevger apparatus. The antimicrobial effects of oil towards Staphylococcus aureus, Pseudomonas aerugenosa, Candida albicans and Aspergillus niger were tested by Inhibition Zone Diameter (IZD) test to screen the antimicrobial activity, Minimum Inhibitory Concentration (MIC) test and Minimum Killing Time (MKT) test to determine the minimum concentration of oil and minimum time required to kill the pathogens. Oil showed very good activity against all four microbial strains used at concentration of 10 μL except Pseudomonas sp. Very low concentration of 1.95 μL mL-1 oil was needed to inhibit the growth of most highly infecting pathogen Staphylococcus aureus within 15 min of its exposure in comparison to other microbial strains. High turmerone content (49.76%) of elite turmeric cultivar Roma released from Orissa (India) might be assigned to be responsible for such excellent anti microbial activity against the tested pathogens.

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