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Research Journal of Medicinal Plants
Year: 2008  |  Volume: 2  |  Issue: 2  |  Page No.: 79 - 85

Cellular Effects of Garlic (Allium sativum) Extract on Pseudomonas aeruginosa and Staphylococcus aureus

B.E. Boboye and A.J. Alli    

Abstract: Effects of garlic extract at 67, 134 and 201 mg mL-1 on Pseudomonas aeruginosa and Staphylococcus aureus were studied. In the absence of the extract, the cells grew to high densities within 11/2 h at 37 °C. Garlic extract-treated cells reduced in number and died. Percentage living cells at 201 mg mL-1 was 0% for both bacteria. Sucrose and MgSO4 stabilized and protected the cells. At 67, 134 and 201 mg mL-1 of the extract in the presence of this sugar and the compound, 47, 4 and 0% of Ps. aeruginosa cells were viable. Microscopic examination of carbol fuschin and Giemsa stained cells showed that the garlic treated cells were bigger in size than those of untreated ones; and intact and definite nuclei were lacking. The cell wall was the target of attack and the extract was bacteriolytic in action.

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