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Research Journal of Medicinal Plants
Year: 2007  |  Volume: 1  |  Issue: 4  |  Page No.: 112 - 120

Role of Terpenoids From Elephantopus scaber Against a Few Extended Spectrum β-Lactamase Producers

R. Jasmine, P. Daisy and B.N. Selvakumar    

Abstract: The extended spectrum β-lactamase producers are highly resistant to several conventional antibiotics. Hence efforts are now taken to screen few medicinal plants against the ESBL producers. Among the several plants screened, we have chosen to screen the alcohol extracts of a traditional medicinal plant, Elephantopus scaber (Asteraceae) against ESBL producers. ESBL producers were screened by double disc synergy test. Methanol, hexane and acetone extracts of Elephantopus scaber were investigated for their ability to inhibit the growth of extended spectrum β-lactamases (ESBL) producing multidrug-resistant enteric bacteria by the disc diffusion method. MICs were determined by micro broth dilution method. The crude plant extracts demonstrated zones of inhibition in the range of 5-16 mm against the chosen test bacteria. On the basis of promising activity, acetone extracts were selected to determine their efficacy in terms of Minimal Inhibitory Concentration (MIC), which ranged from 1.6-25 mg mL-1. The acetone extract was subjected to activity-guided fractionation. The most effective fraction had a MIC of 62.5-250 μg mL-1. Phytochemical analysis showed the presence of terpenoids, proteins and traces of steroids. TLC bioautography of the fraction showed the active compound to be terpenoids. The fraction was further tested for their in vivo cytotoxic activity to mammalian system using rats. No marked manifestations were observed. Normal liver and kidney functioning were also observed. The strong in vitro antibacterial activity of terpenoid derivatives against ESβL-producing Gram-negative bacteria suggests the compounds might find wide pharmaceutical use.

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