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Research Journal of Environmental Toxicology

Year: 2009  |  Volume: 3  |  Issue: 4  |  Page No.: 159 - 169

Evaluation of Alcaligenes faecalis Degradation of Chrysene and Diesel Oil with Concomitant Production of Biosurfactant

M.N. Igwo-Ezikpe, O.G. Gbenle, M.O. Ilori, J. Okpuzor and A.A. Osuntoki

Abstract

Alcaligenes faecalis was evaluated for its potential to degrade varying concentrations of chrysene and diesel oil with concomitant biosurfactant production. Biodegradation was set up for 7 days utilizing the substrates as sole carbon and energy sources. Residual chrysene obtained after degradation of 30, 50 and 100 mg L-1, respectively was 17.4±1.5, 27.2±1.2 and 28.7±1.4 mg L-1 while total petroleum hydrocarbon remaining after degradation of 3, 5, 15 and 30% (v/v) diesel oil respectively was 2.58±0.5, 3.09±1.2, 21.65±5.4 and 63.92±8.1%. Microbial cells of A. faecalis and sterilized cell-free extract from diesel oil media showed emulsifying activities against kerosene, diesel oil, engine oil, hexadecane, dodecane, xylene and hexane whereas no emulsifying activity was observed of microbial cells and sterilized cell-free extract from chrysene media. Alcaligenes faecalis cells harvested from diesel oil media also showed haemolytic activity unlike the microbial cells from chrysene media. Growth of the isolate in chrysene and diesel oil media induced secretion of protein and carbohydrate into the media which were statistically significantly (p<0.05) different compared to controls. This study portrays the potential of Alcaligenes faecalis to degrade and grow on chrysene and diesel oil and induce extracellular protein and carbohydrate with concomitant production of biosurfactant for industrial purposes and in hydrocarbon bioremediation.

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