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Research Journal of Environmental Sciences
Year: 2011  |  Volume: 5  |  Issue: 5  |  Page No.: 479 - 485

Activity and Stability of Herbicide Treated Cyanobacteria as Potential Biomaterials for Biosensors

A. Tripathi, S. Sundaram, B.C. Tripathy, B.S. Tiwari and A. Rahman    

Abstract: In present study we tried to improve the sensitivity of whole cells of cyanobacteria, spheroplasts and thylakoidal fragments of cyanobacteria in which PSII is the main target for most of the herbicides, some heavy metals and organic pollutants. We also screened out the potential of tolerance of different cyanobacterial species for herbicides. These biologically active materials are capable of oxygen evolution by photolysis of water in the presence of light. We performed growth study, chlorophyll a analysis, bioenergetic study, oxygenevolution property using clark’s type oxy electrode for the comparison of activity both in free cell stage and immobilized condition. We recorded the changes in their spectral properties, oxygen evolution and chlorophyll a concentrations in the presence of various herbicides. We determined the I50 values for whole cells of different cyanobacterial species and also recorded peak shifting.

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