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Research Journal of Environmental Sciences

Year: 2010  |  Volume: 4  |  Issue: 1  |  Page No.: 50 - 56

Genetic Diversity in Ecotypes of Two Agropyron Species using RAPD Markers

A. Arghavani, A. Asghari, M. Shokrpour and Mohammaddost Chmanabad

Abstract

This study conducted to analyze genetic diversity in two Agropyron species, A. pectiniforme and A. elongatum, by RAPD analysis. Random Amplified Polymorphic DNA (RAPD) analysis using 12 primers produced 142 polymorphic bands with lengths ranging 564 to 2,000 bp. On the basis of Nei's gene index, the genetic diversity within ecotypes varied from 0.1014 to 0.178. The highest and lowest of this index were obtained in ecotype of 6951 (from A. pectiniforme; 0.178) and ecotype of 225 (from A. elongatum; 0.1014), respectively. The results showed high variation within ecotypes. The average gene diversity within (HS), total (HT) and coefficient of gene differentiation (GST) were 0.13, 0.27 and 0.47, respectively. Analysis of molecular variance (AMOVA) displayed significant variance within and among Agropyron ecotypes. The variance within ecotypes (61.08) was about two times higher than between ecotypes (31.24). Cluster analysis based on RAPD data using Nei's genetic distance categorizes the entries into four clusters. Using principle coordinate analysis, the first three coordinates accounted for the 52.84% of the total variation. Classifying the ecotypes by the two coordinates verified the results of cluster analysis.

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