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Research Journal of Environmental Sciences
Year: 2009  |  Volume: 3  |  Issue: 2  |  Page No.: 251 - 256

Production of Mannan-Degrading Enzymes from Aspergillus niger and Sclerotium rolfsii Using Palm Kernel Cake as Carbon Source

S. Abd-Aziz, N.A. Ab-Razak, M.H. Musa and M.A. Hassan    

Abstract: The aim of this study is to produce and profile the mannan-degrading enzymes from local fungal isolates by submerged fermentation and saccharification of PKC. Results showed that Aspergillus niger and Sclerotium rolfsii can produce mannan-degrading enzymes. The fungi were grown in submerged fermentation of PKC to produce mannan-degrading enzymes. The highest alpha-galactosidase was obtained on day 13 of fermentation (0.128 U mL-1) when using A. niger and on day 18 (0.126 U mL-1) when using S. rolfsii. Analysis also showed that enzyme activities for beta-mannanase using S. rolfsii were the highest at day 17 (3.166 U mL-1) and for A. niger (2.482 U mL-1) at day 8. Meanwhile the highest beta-mannosidase were obtained at day 16 for A. niger (0.128 U mL-1) and for S. rolfsii at day 16 (0.116 U mL-1).

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