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Research Journal of Dairy Sciences
Year: 2014  |  Volume: 8  |  Issue: 4  |  Page No.: 34 - 40

Early Detection of Aspergillus fumgitus in a High Risk Group of Dairy Cattle using Nested and Real-Time Quantitative PCR

Liangcheng Chen, Tao Jiang, Xiao Li, Qi Wang, Yu Li, Kevin Marley and Yongqiang Wang    

Abstract: Early detection of Aspergillus fumigatus (A. fumigatus) may facilitate early diagnosis and prevention of Invasive Aspergillosis (IA) in dairy cows. This study used a nested PCR and real-time quantitative PCR method to measure A. fumigatus in feed, tissue and blood samples collected from domestic dairy farms in China. In total, 396 feed samples from across China including corn, soybean meal, cotton seed meal, wheat mill run, distiller’s dried grains, corn and grass silage and Total Mixed Ration (TMR) were tested. Of these, 95% contained detectable levels of A. fumigatus. Blood and tissue samples from the GI tract of 7 unhealthy cows all tested positive for A. fumigatus. The analytical sensitivity of the PCR method was<1 fg/assay.

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