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Research Journal of Biological Sciences
Year: 2013  |  Volume: 8  |  Issue: 4  |  Page No.: 88 - 93

Detection of Metallo-β-Lactamase (MBL) Producing Acinetobacter at 3 Hospitals in Iran, Tehran

Y. Erfani, M. Farahbakhsh, H. Godarzi, G. Eslami, A. Hashemi, S. Alimehr, Hakemi Vala and A. Rasti    

Abstract: Acinetobacter sp. can cause serious health care-associated infactions. Metallo-β-Lactamase (MBL) producing acinetobacters (MBL) are responsible for worldwide nosocomial infections. Acinetobacter species almost are resistant to all antibiotics Multi-Drug-Resistant (MDR). Therefore, detection of these organisms is very important. This study was carried out on 107 isolate of acinatobacter from patients admitted in 3 hospitals in Tehran (August, 2012 to May, 2013). Antimicrobial susceptibility test was performed by Kirby-Bauer method (disc diffusion agar or DDA) for: Amikacin (30 μg), imipenem (10 μg), meropenem (10 μg), piperacillin (100 μg), p-tazobactam (110 μg), cefotaxime (30 μg), ceftazidime (30 μg), cefepime (30 μg), ciprofloxacin (5 μg), tetracycline (30 μg), ticarcillin (75 μg), tobramycin (10 μg), gentamicin (120 μg), cotrimoxazole (25 μg) and levofloxacin (5 μg). In Antimicrobial Susceptibility Test (AST), 100% of strains were resistant to cefotaxime, 98.13% to cotrimoxazole, 97.19% to piperacillin, cefepime and ticarcillin, 96.26% top-tazobactam, 95.3% to imipenem, ceftazidime and ciprofloxacin, 94.3% to meropenem, 93.4 to levofloxacin, 87.5% to amikacin, 64.4% to tobramycin, 61.68% to tetracyclin and 51.4% to gentamicin. All tests were performed on muller hinton agar. Later MIC of colistin was performed according to CLSI protocol . That showed 93.5% sensitivity of isolates. MBLs was detected by CDDT (combind disc diffusion agar method). Finaly, the results releved that 92.5% of imipenem and meropenem rasistant acinetobacter were MBL positive (showed significant zone around imipenem-EDTA disc) that is alarming and indicates necessity of proper planning for antibiotic prescription and the prevention of overuse antibiotic consumption in order to prevention of spreading these important resistant bacteria.

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