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Research Journal of Biological Sciences
Year: 2013  |  Volume: 8  |  Issue: 2  |  Page No.: 54 - 61

Influence of Physical and Chemical Parameters on Protease Production by Bacillus megaterium IBRL MS 8.2 in Submerged Fermentation

I. Darah, M.L. Choo and S.H. Lim    

Abstract: Proteases are a group of enzymes that catalyze the hydrolysis of protein and among the various proteases, bacterial proteases are the most significant when compared to animal and fungal proteases. In this study, a local bacterial isolate Bacillus megaterium IBRL MS 8.2 was found to produce the highest protease production of 155.38 U mL-1 when cultivating it in the improved medium which consisted of initial medium pH of 7.5, inoculum size of 7.5% (3x107 cells mL-1), agitation speed of 150 rpm, incubation temperature of 30°C, 0.5% of sucrose and 1.0% of beef extract. The highest protease production was achieved on the 30 h of cultivation. On the other hand, the highest cell growth of 2.33 g L-1 was achieved on 18 h of cultivation time. The data obtained suggest that enzyme production by B. megaterium IBRL MS 8.2 was not growth dependant.

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