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Research Journal of Biological Sciences

Year: 2011  |  Volume: 6  |  Issue: 8  |  Page No.: 345 - 348

Contamination Rate of Marketed Raw Milk in Tabriz City to Coagulase Positive Staphylococcus aureus by Culture and PCR Methods

Mansoor Khakpoor and Saeid Safarmashaei


Milk has an outstanding nutritional quality but is also an efficient vehicle for transmission of diseases to humans and is an excellent medium for bacterial growth and an important source of bacterial infection when consumed without pasteurization. S. aureus is an important pathogen due to a combination of toxin-mediated virulence, invasiveness and antibiotic resistance. Milk is a good substrate for S. aureus growth and enterotoxin production. The aim of this study was to determination of contamination ate of raw milk to Staphylococcus aureus by Culture and PCR Method in Tabriz city. A total of 100 raw milk samples were collected from the milking bucket into a plastic or aluminum containers which were not well washed, no cooling system was applied at any level of the milk chain which may last for 5 h till milk reaches the consumer. The collected milk samples were cultured on selective media and identification of the isolated colonies identified by PCR Method. In this study, 30% raw milk contaminated to coagulase positive Staphylococcus aureus were detected. It can be concluded that raw milk is contaminated by this pathogen in this area as well as in other countries and might constitute a risk for S. aureus enterotoxin food poisoning.