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Research Journal of Biological Sciences
Year: 2011  |  Volume: 6  |  Issue: 3  |  Page No.: 118 - 123

Antioxidant Enzyme Activities Characterization in Pulmonary Hypertension Syndrome (PHS) in Broilers

M. Fathi, K. Nazeradl, Y. Ebrahim Nezhad, H. Aghdam Shahryar, M. Daneshyar and T. Tanha    

Abstract: Major objectives of this study were to assess antioxidant protection in plasma and whole liver in broilers with pulmonary hypertension syndrome. The experiment was conducted with 160, 1 day old male broilers (Ross 308). The chickens were divided in 2 groups of 4 replicates and 20 chicks for any replicate. One group of these chickens was raised in normal temperature (control without PHS) treatment and the other group was raised in cold temperature (with PHS) treatment for induce pulmonary hypertension syndrome. Mortality was inspected to determine cause of death and diagnose of PHS. For evaluation of antioxidant status, the parameters that were determined in plasma and liver include: Glutathione Peroxidase (GPX), Superoxide Dismutase (SOD), Total Antioxidant Status (TAS) and Malondialdehyde (MDA). Sampling of blood and liver tissue were determined at day 21 and 42. At end of the experiment (week 6), 2 chicks from each replicate were randomly selected and slaughtered. The heart was removed, the right ventricle was dissected away from the left ventricle and septum then ratio of Right Ventricle weight to Total Ventricle weight (RV/TV) calculated too. The results of the experiment indicated that in plasma (GPX) and (SOD) activity were elevated in broilers with PHS compared to controls. It was also, PHS group had low TAS compared to control. But in liver, just GPX (not for SOD) was elevated in PHS group than control. The levels malondialdehyde equivalents an indicator of lipid oxidation subsequent to generated oxidative stress at plasma and liver tissue was significantly higher in PHS group compared control. RV/TV ratio and mortality due to ascites, also were significantly affected by treatments as PHS group had greater RV/TV and mortality due to ascites mortality percentage compared to control.

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