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Research Journal of Biological Sciences
Year: 2010  |  Volume: 5  |  Issue: 4  |  Page No.: 310 - 313

Antioxidative Activities and Phenolic Content of Extracts from Okra (Abelmoschus esculentus L.)

Panadda Khomsug, Walaiporn Thongjaroenbuangam, Noppakun Pakdeenarong, Maitree Suttajit and Piyanete Chantiratikul    

Abstract: The aim of this study was to determine the antioxidant activities, TPC and to identify phenolic compounds of seeds and pulped okra (Abelmoschus esculentus L.), a vegetable that is consumed worldwide because it plays an important role in the human diet by supplying carbohydrates, minerals and vitamins. The antioxidant activities of seeds and pulped okra were evaluated by DPPH radical scavenging and ABTS radical cation decolorization assays were used. Total Phenolic Content (TPC) was determined using the folin-ciocalteu assay. I dentification of phenolic compounds was achieved using HPLC with the UV-diode array detection. The TPC of pulped and seeds of okra extracts were 10.75±0.02 mg GAE/100 g e xtract and 142.48±0.02 mg GAE/100 g extract which corresponding with scavenging activities. The predominant phenolic compound was procycanidin B2, following procycanidin B1 and rutin in seeds but in pulped okra they were identified as catechin, procycanidin B2, epicatechin and rutin. These findings in associations with the high content of antioxidant activity and phenolics in okra enhance the importance of this food stuff for the human diet.

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