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Research Journal of Biological Sciences

Year: 2009  |  Volume: 4  |  Issue: 10  |  Page No.: 1073 - 1075

Analysis of Kappa Casein Gene Polymorphism by PCR-RFLP in Buffalo Population in Khouzestan Province

B. Abbasi, J. Fayazi, M.T. Beigi Nasiri, H.A. Roshanfekr, Kh. Mirzadeh and A.S. Sadr


Caseins are a family of milk proteins that approximately constitute 80% of total milk proteins. They are existing in several molecular forms (alpha S1, alpha S2, beta and kappa) with variant alleles of each. The A and B are the most common in cattle breeds. Allele A has threonine (ACC) and aspartic asid (GAT) amino acid at position 136 and 148, respectively by means of point mutation, isoleucine (ATC) substitutes threonine and aspartic acid is substituted by alanine (GCT) and variant A changes to variant B. In this study, the PCR-RFLP method was used for differentiating buffalo population in Khouzestan Province k-CN allels. In order to determaind the level of polymorphism, blood sample were collected from 86 buffalos in Shadegan, Ahwaz, Sosangerd, Dezfoul and Shoshtar cities. DNA extraction was based on Boom method and exon 4 of the kappa casein gene was amplified by specific set of primers for this gene to produce a 453 bp fragment. The amplified fragment were digested with Hinf I restriction endonuclease and then subjected to electrophoresis separation in ethidium bromide-stained 2.5% agarose gel. The results were revealed all buffalo samples were monomorph and genotyped as BB. In this breed, the B allele is only existed, which is reported to be favorable for milk quality and can use for breeding strategies of dairy animals.