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Research Journal of Biological Sciences
Year: 2007  |  Volume: 2  |  Issue: 1  |  Page No.: 91 - 95

PCR-SSCP Analysis of Avian Lipoprotein Lipase Gene (Intron 8) and its Relation with Body Weight and Abdominal Fat

H.H. Musa, G.H. Chen, A.H. Suleiman and D.M. Mekki    

Abstract: The genetically fat Anka and lean Rugao chicken breeds were used for this study, both are reared in the same environment and management system. Body weight and abdominal fat weight were determined at 12 weeks of age. A polymerase chain reaction single strand conformation polymorphism technique was applied to detect mutation in intron 8 of lipoprotein lipase gene. Hardy Weinberg Equilibrium (HWE) test shows that allele frequency was significantly (p<0.01) different in Rugao population in locus LPL10 and non significantly different in Anka population. In addition chi-square test shows that populations were significantly (p<0.01) differed from each other. Body weight in fat breed was significantly (p<0.05) different between LPL genotypes in LPL9 and LPL10 loci. However, the abdominal fat was significantly (p<0.05) different in LPL10 locus. In addition lean breed shows non significant difference on body weight and fat weight. Genotype effect within two breeds showed that body weight and fat weight in LPL9 and LPL10 loci were significantly (p<0.05) higher in heterozygous AC compared with homozygous AA.

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