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Research Journal of Biological Sciences
Year: 2007  |  Volume: 2  |  Issue: 2  |  Page No.: 112 - 117

Antioxidant Activity in Cotyledon of Black and Yellow Common Beans (Phaseolus Vulgaris L.)

N.E. Rocha-Guzman, J.A. Gallegos-Infante, R.F. Gonzalez-Laredo, A.M. Preza Y. Lerma and F.J. Ibarra-Perez    

Abstract: Methanol and aqueous acetone cotyledon extracts from two groups of different colored common beans (Phaseolus vulgaris L.), black and yellow, were analyzed for total phenolics, lipid antioxidant activity and two-dimensional thin layer chromatography. A higher yield was obtained for methanol extracts in comparison to acetone extracts. However, acetone extracts showed a higher phenol content than methanol extracts independently of the group of beans tested. Furthermore, phenolic content was higher in acetone extracts from black beans than in acetone extracts of yellow beans. A higher antioxidant activity was found in methanol extracts rather than in acetone extracts without differences in the group of beans studied. Most of antioxidant activity was associated to black beans. Thin layer chromatography showed the presence of catechins in black beans but not in yellow beans cotyledons.

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