Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Research Journal of Botany
Year: 2012  |  Volume: 7  |  Issue: 1  |  Page No.: 1 - 7

Standardizing Sterilization Protocol and Establishment of Callus Culture of Sugarcane for Enhanced Plant Regeneration in vitro

Satish Tiwari, Arvind Arya and Sandeep Kumar    

Abstract: The present investigation is an attempt towards finding an effective sterilization protocol for two, field grown sugarcane varieties, CoS 88230 and CoS 767 from Meerut region and to establish the callus culture protocol. Four sterilizing agents EtOH, NaOCl, HgCl2 and H2O2 were used alone and in combination. Our finding suggests that the use of only one sterilizing agent is not successful for the sterilization of leaf sheath explant of sugarcane. HgCl2 (0.1%) for 5 min and EtOH (90%) for 10 min was found best when used along with prior washing and surface sterilization with 20 and bavistin. An increased response of callus initiation was achieved on MS medium supplemented with 2,4-D (1 mg L-1) and antibiotic amoxicillin (2 mL L -1). present research attempt will allow mass multiplication of economically important sugarcane varieties through tissue culture technology can be used further to complete the demand of farmer for the planting material of the genetically superiors sugarcane varieties.

Cited References   |    Fulltext    |   Related Articles   |   Back
  Related Articles

Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility