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Research Journal of Botany
Year: 2011  |  Volume: 6  |  Issue: 1  |  Page No.: 1 - 10

Study of Different Stages of Somatic Embryogenesis in a Medicinal Plant, Madar (Calotropis procera)

S. Sundaram, I.Z. Ahmad and P. Dwivedi    

Abstract: An efficient protocol for in vitro regeneration of endangered medicinal plant Calotropis procera has been developed. The juvenile leaf explants were transferred to MS medium containing different combinations of PGRs. Among the various combinations of Kinetin (1.0-3.0 mg L-1) and 2,4-D (1.0-5.0 mg L-1) the intensity of callus induction was highest in MS medium with Kinetin (1 mg L-1) and 2, 4-dichloro phenoxy acetic acid (3 mg L-1). The percentage of calli forming embryos was 82.6. During organogenic callus formation, different types of calli with variation in color and texture were noticed and among them, the light green, fragile calli responded well for the induction of shoots. Among the various combinations of BAP and IAA used the differentiation was seen to be the best in the presence of 2 mg L-1 BAP and 1 mg L-1 NAA frequency of shoot regeneration was highest 75%. The shoot was transferred to MS media for root regeneration containing PGRs BAP (1-4) +NAA (1-4) mg L-1. The frequency of root regeneration was 100% in MS medium containing BAP (1) + NAA (4) mg L-1. The protocol was optimized by manipulations of different PGRs for enhanced multiplication. Protocol explained in this research paper provides a rapid plant regeneration system which could be used for the somaclonal variation; shoot induction and producing transgenic plants in Calotropis.

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