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Research Journal of Botany
Year: 2007  |  Volume: 2  |  Issue: 4  |  Page No.: 195 - 201

Somatic Embryogenesis in Two Varieties of Eggplant (Solanum melongena L.)

A.K.M.N. Huda, M.A. Bari, M. Rahman and N. Nahar    

Abstract: This research programme was undertaken with a view to develop an efficient and reliable method of indirect regeneration through somatic embryogenesis in two varieties of eggplant through the technique of tissue culture. For this purpose, cotyledon pairs of Solanum melongena were used as explants for callus induction. Three different auxins (NAA, 2, 4-D and IAA) were used singly or in combination with BAP for present investigation, as types, concentrations and combinations of growth regulators had marked influence on callus induction. Auxin/cytokinin combinations were proved more potent for callus induction as well as callus growth than that of auxin tried singly in the media. Best callus formation was observed in MS media when supplemented with 0.05 mg L-1 BAP + 2.0 mg L-1 NAA. Best calli from cotyledonary explant were subcultured on MS supplemented with different combinations and concentrations of phytohormones for somatic embryogenesis. Among the different combinations and concentrations 0.05 mg L-1 BAP + 2.0 mg L-1 NAA gave the best result on somatic embryogenesis for Loda variety. On the other hand, somatic embryos of China variety were obtained only on the media having 1.0 mg L-1 BAP + 0.05 mg L-1 GA3. The global structure somatic embryos originated from the superficial layers of the calli, as a single structure or in a cluster. These structures could be isolated easily from parent tissues because they were floated freely in water. These floated free embryos were cultured on nutrient media again to obserb shoot primordia development and thereafter shoot development.

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