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Research Journal of Botany
Year: 2007  |  Volume: 2  |  Issue: 2  |  Page No.: 102 - 107

An Efficient Micropropagation System for Vitex negundo L., an Important Woody Aromatic Medicinal Plant, Through Shoot Tip Culture

P.K. Usha, Sailas Benjamin, K.V. Mohanan and A.V. Raghu    

Abstract: An efficient protocol was established for in vitro shoot multiplication from shoot tip explants of Vitex negundo on Murashige and Skoog (MS) basal medium supplemented with 8.87 μM 6-benzylaminopurine (BA). Inclusion of 8-Naphthalene Acetic Acid (NAA) in the culture medium along with BA promoted higher rates of shoot multiplication than BA alone. The rate shoot multiplication (6.3) after 4 week of culture on MS basal medium supplemented with 8.87 μM BA, 2.69 μM NAA. The elongated shoots rooted in half strength MS basal salts supplemented with 4.90 μM IBA + 2.85 μm IAA and 2% (w/v) sucrose. The presence of Activated Charcoal (AC) with IBA showed positive response to rooting. In vitro propagated plants were transferred to soil with a survival rate of 85% after 1 month.

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