Abstract: Evaluation of the antagonistic activity of three bacterial and a fungi with direct confrontation method and the filtrates culture against the growth of Fusarium oxysporum f.sp. lycopersici showed the inhibition of the mycelia growth of Fusarium oxysporum f.sp. lycopersici with Bacillus cereus energized the low activity and it was more significant with Serratia marcescens and Trichoderma harzianum for the 2nd day but with Pseudomonas fluorescens, it was for the 5th day. The filtrates of culture of these antagonists showed that only Serratia marcescens and Trichoderma sp. have a rate of inhibition which varies between (40-95%) and of (20-30%) with Pseudomonas fluorescens.