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Pakistan Journal of Nutrition
Year: 2020  |  Volume: 19  |  Issue: 3  |  Page No.: 111 - 116

High Fiber Diet Reduces Gene Expression and Level of IL-1β in Hypertriglyceridemia Rats

Danus Hermawan, Pramudji Hastuti and Sunarti    

Abstract: Background and Objective: High fat and fructose diet causes hypertriglyceridemia that induce production of proinflammatory interleukin Iβ (IL-Iβ). Expression of IL-Iβ can be suppressed by short chain fatty acids (SCFA).This study aimed to determine the effects of high fiber diet on the level and expression of IL-1β in high fat and fructose diet model of Wistar rats. Materials and Methods: Twenty-five Wistar rats divided into 5 groups: Normal control (N), hypertriglyceridemia control (TC), hypertriglyceridemia with 1.04 g rat–1 day–1 of fiber (T1); hypertriglyceridemia with 2.07 g rat–1 day–1 of fiber (T2) and hypertriglyceridemia with 3.11 g rat–1 day–1 of fiber (T3). Triglyceride levels were measured using colorimetric method, whereas IL-1β levels were measured using ELISA method. All biochemical analysis was performed twice, before and after treatment. IL-1β gene expression in white adipose tissue was measured by q-PCR method at the end of the study. Results: Triglyceride levels in treatment groups were lower compared to hypertriglyceridemia control group. Gene expression and levels of IL-1β at treated groups, receiving high fiber diet, were lower compared to the hypertriglyceridemia control group. Conclusion: High fiber diet could suppress the expression of IL-1β in white adipose tissues and could reduce the level of IL-1β in rats.

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