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Pakistan Journal of Nutrition
Year: 2017  |  Volume: 16  |  Issue: 8  |  Page No.: 571 - 576

Increasing the In Vitro Enzymatic Activity of Cellulase and Amylase from Beef Cattle Rumen Fluid Supplemented with Moringa oleifera Leaves and Sulfur

F.M. Suhartati and W. Suryapratama    

Abstract: Background and Objective: A combination of feed with Moringa oleifera can be used for the optional development of rumen microbes which will provide th protein to host animal. An in vitro study was conducted to assess the activity of cellulases, protease and amylase enzymes from beef cattle rumen fluid supplemented with Moringa oleifera leaves and sulfur. Methodology: The experiment used a Completely Randomized Design (CRD) of 3×2 variables including three concentrations of Moringa oleifera leaves (0, 10 and 20%) and two levels of sulfur supplementation (0 and 0.4%), producing six treatments. Each treatment was replicated four times for a total of 24 experimental conditions involving 4 h of in vitro incubation each. Rumen fluid derived from bulls was sourced immediately after slaughter. Moringa oleifera leaves were obtained from Kutasari village. The activities of cellulases, protease and amylase enzymes were measured. Results: The results demonstrated that the interaction between Moringa oleifera leaves and supplemental sulfur significantly increased (p<0.05) cellulases activity and highly significantly increased (p<0.01) amylase activity but had no significant effect (p>0.05) on protease activity. Increased supplementation with Moringa oleifera leaves decreased protease activity. Conclusion: The supplementation of bovine rumen fluid with 20% Moringa oleifera leaves and 0.4% sulfur resulted in the highest measured activities of cellulases and amylase.

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