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Pakistan Journal of Nutrition
Year: 2017  |  Volume: 16  |  Issue: 1  |  Page No.: 22 - 27

Effects of Bacterial Inoculants and Enzymes on the Fermentation, Aerobic Stability and in vitro Organic Matter Digestibility Characteristics of Sunflower Silages

Mehmet Levent Ozduven, Fisun Koc and Veysel Akay    

Abstract: Objective: This study was carried out to determine the effects of lactic acid bacteria inoculant, enzymes and lactic acid bacteria inoculant+enzymes mixture on the fermentation, cell wall content, aerobic stability and in vitro organic matter digestibility characteristics of sunflower silages. Methodology: Sunflower was harvested at the milk stage of maturity. The treatments were as follows: (1) Control (no additive), (2) Inoculation of lactic acid bacteria (LAB, 2 g t–1, a mixture of Lactobacillus plantarum and Enterococcus faecium applied at a rate of 6.00 log10 CFU LAB g–1 of fresh material) (3) Enzyme (E, 150000 CMCU kg–1 for cellulase and 200000 SKB kg–1 for amylase) and (4) LAB+enzyme mixture (LAB+E, 2 g t–1 a mixture of Lactobacillus plantarum bacterium (6.00 log10 CFU g–1) and 150000 CMCU kg–1 for cellulase and 200000 SKB kg–1 for amylase). After treatment, the chopped sunflower was ensiled in 1.0-l special anaerobic jars, equipped with a lid enabling gas release only. The jars were stored at 25±2°C under laboratory conditions. Three jars from each group were sampled for chemical and microbiological analysis for 2, 4, 8 and 60 days after ensiling. At the end of the ensiling period all silages were subjected to an aerobic stability test for 5 days. Results: In addition, in vitro organic matter digestibilities of these silages were determined. Both inoculants (LAB and LAB+E) increased characteristics of fermentation but impaired aerobic stability of sunflower silages. Lactic acid bacteria+enzymes mixture inoculants decreased neutral and acid detergent fiber content and than control silages. Conclusion: In vitro organic matter digestibility was numerically increased for treated than control silages.

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