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Pakistan Journal of Nutrition
Year: 2012  |  Volume: 11  |  Issue: 1  |  Page No.: 88 - 93

Production of Lactic Acid by a Local Isolate of Lactobacillus plantarum Using Cheap Starchy Material Hydrolysates

Amal Kadhim G. Al-Asady    

Abstract: Some cheap starchy materials like sorghum grains and wheat bran were degraded by crude glucoamylase of a local isolate of Mucor sp., then Lactic Acid (LA) was produced by a local isolate of L. plantarum using sorghum flour hydrolysate, sorghum starch hydrolysate, soluble starch hydrolysate, wheat bran hydrolysate and date syrup with 10% reducing sugars. The yield of LA increased to 37.2 g/100 reducing sugars by using sorghum flour hydrolysate as a basal medium supplemented with (0.6+0.6)% yeast extract+(NH4)2HPO4 and 0.06% MgSO4.7H2O, reducing sugars was 5%. The fermentation temperature was 30°C/96 h. Results indicated that using hydrolysates mixtures of sorghum flour and wheat bran improved LA fermentation. The yield of LA was 92.5 g by using sorghum flour and 50% wheat bran. Paper chromatography indicated that LA was the unique organic acid in the fermented broth.

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