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Pakistan Journal of Biological Sciences
Year: 2015  |  Volume: 18  |  Issue: 5  |  Page No.: 232 - 239

Comparison of Antioxidant Activity of Rheum ribes Fruits and Seed Methanolic Extracts against Protein Oxidation and Lipid Peroxidation

Isıl Yildirim, Turkan Kutlu and Kasım Takim    

Abstract: This work was performed to comparison antioxidant activity of Rheum ribes fruit and seed methanolic extracts against to protein oxidation. Consequently determined that antioxidant capacity with cupric of method, respectively in fruit extract to be 2.44±0.212, in seed to be extract 5.30±0.245 mmol g–1, antioxidant radical scavenging with ABTS* method in fruit extract to be 11-78%, in seed extract 2-15% levels. The OH. power reduction for the Rheum ribes fruit extract 34-49, 22±0.04%, while seed extract to be 49-80±0.04%, H2O2 removal activity for fruit extract was at 30.31-57.87 %, while seed extract was at %33.33-54.57 levels. Also were determined the total phenolic content, respectively in fruit extract to be 2.312±0.0007 in seed extract to be 3.298±0.0007 mg of GA equivalent/g, total flavonoid content in fruit extract to be 2.79±0.0066, in seed extract to be 7.31± mg 0.1787 of Q equivalent/g, total anthocyanin content, respectively in fruit extract to be 0.5 mg of cyanidin-3-glucoside equivalent/L, in seed extract to be 1.26 mg of cyanidin-3-glucoside equivalent/L. were found. Protein oxidation in fruit was 40.64-56.5%, in seed extract it was 37.98-51.46% were determined. Anti-lipid peroxidation inhibition % in fruit extract to be 13.72-27.4%, in seed extract to be 8.459-27.09% was determined.

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