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Pakistan Journal of Biological Sciences
Year: 2011  |  Volume: 14  |  Issue: 9  |  Page No.: 533 - 539

Optimization of Temperature, Moisture Content and Inoculum Size in Solid State Fermentation to Enhance Mannanase Production by Aspergillus terreus SUK-1 using RSM

Jahwarhar Izuan Abdul Rashid, Noraini Samat and Wan Mohtar Wan Yusoff    

Abstract: Optimization of three parameters, temperature (25-35°C), moisture content (40% (w/v)-60% (w/v) and inoculum sizes (5% (w/v)-15% (w/v) were investigated and optimized by Response Surface Methodology (RSM) for optimal mannanase production by Aspergillus terreus SUK-1. A second order polynomial equation was fitted and the optimum condition was established. The result showed that the moisture content was a critical factor in terms of its effect on mannanase. The optimum condition for mannanase production was predicted at 42.86% (w/v) initial moisture (31°C) temperature and 5.5% (w/v) inoculum size . The predicted optimal parameter were tested in the laboratory and the mannanase activity 45.12 IU mL-1 were recorded to be closed to the predicted value (44.80 IU mL-1). Under the optimized SSF condition (31°C, 42.86% moisture content (w/v) and 5.5% inoculum size (w/v)), the maximum mannanase production was to prevail about 45.12 IU mL-1 compare to before optimized (30°C, 50% moisture content (w/v) and 10% inoculum size (w/v)) was only 34.42 IU mL-1.

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