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Pakistan Journal of Biological Sciences
Year: 2011  |  Volume: 14  |  Issue: 3  |  Page No.: 226 - 231

Production and Partial Characterization of Uric Acid Degrading Enzyme from New Source Saccharopolyspora sp. PNR11

K. Khucharoenphaisan and K. Sinma    

Abstract: The strain PNR11 was isolated from gut of termite during the screening for uric acid degrading actinomyces. This strain was able to produce an intracellular uricase when cultured in fermentation medium containing uric acid as nitrogen source. Base on its morphological characters and 16S rDNA sequence analysis, this strain belong to the genus Saccharopolyspora. This is the first report of uricase produced from the genus Saccharopolyspora. The aim of this study was to investigate the effects of different factors on uricase production by new source of Saccharopolyspora. Saccharopolyspora sp. PNR11 was cultured in production medium in order to determine the best cultivation period. The result showed that the time period required for maximum enzyme production was 24 h on a rotary shaker operating at 180 rpm. Optimized composition of the production medium consisted of 1% yeast extract, 1% maltose, 0.1% K2HPO4, 0.05% MgSO4 7H2O, 0.05% NaCl and 1% uric acid. The optimum pH and temperature for uricase production in the optimized medium were pH 7.0 and 30°C, respectively. When the strain was cultured at optimized condition, the uricase activity reached to 216 mU mL-1 in confidential level of 95%. The crude enzyme had an optimum temperature of uricase was 37°C and it was stable up to 30°C at pH 8.5. The optimum pH of uricase was 8.5 and was stable in range of pH 7.0-10.0 at 4°C. This strain might be considered as a candidate source for uricase production in the further studies. Present finding could be fulfill the information of uricase produce from actinomycetes.

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