Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Pakistan Journal of Biological Sciences
Year: 2008  |  Volume: 11  |  Issue: 6  |  Page No.: 950 - 952

Callogenic Studies of Achyranthes aspera Leaf Explant at Different Hormonal Combinations

Sara Kayani, Muhammad Zia, Sadia Sarwar, Riaz -ur-Rehman and M. Fayyaz Chaudhary    

Abstract: With the objective to promote in vitro callus induction, leaf segments of Achyranthes aspera were inoculated on basal MS medium supplemented with 3.0% sucrose and 0.8% agar with different concentrations of 2,4-D alone and in combination with NAA, BAP, IAA, IBA and Zeatin. The explants were maintained in growth room at 25 ±1 °C and 16 h light cycle. The best callus induction was obtained with 2,4-D (1.0 and 2.0 mg L-1) in combination with NAA (0.5 mg L-1). Callus induction and good texture from leaf explant was also observed at 2,4-D with BAP. On these combinations morphologically, light green, soft, compact and non-embryogenic callus (Type III callus) was observed. While morphology of callus and callogenic response was poor at 2,4-D alone or in combination with other hormones at different concentrations.

Cited References   |    Fulltext    |   Related Articles   |   Back
   
 
 
 
  Related Articles

 
 
 
 
 
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility